Fig. 6. Endogenous PTEN is distributed homogeneously in the cytosol of HL60 cells. (A) Immunofluorescence analysis with anti-PTEN antibody (red) to show the endogenous PTEN location in unstimulated or fMLP-stimulated HL60 cells. The cells are representative of 25 out of 25 cells analysed. Bar, 10 µm. (B) Immunofluorescence of mock- and PTEN-GFP-transfected CEM cells was performed to demonstrate anti-PTEN antibody (red staining) specificity. Bar, 10 µm. (C) Analysis of PTEN and p85 in the membrane and cytosolic fractions of fMLP-stimulated HL60 cells was performed as described in Fig. 5.