Fig. 7. Intracellular localization of EGFP-FAD24 fusion protein. (A) Intracellular localization of FAD24. NIH-3T3 cells were transiently transfected with an EGFP-FAD24-expressing plasmid or empty vector. One day after transfection, the cells were fixed and stained with DAPI. EGFP signals were detected with a fluorescence microscope. Bar, 25 µm. (B) Colocalization of FAD24 with a nuclear speckle marker. HeLa cells transiently transfected with EGFP-FAD24 or EGFP-truncated FAD24 (FAD24 bZIP), which lacks a bZIP-like domain, were fixed and stained with the nuclear speckle marker SC35. The fluorescence of FAD24 (green) and SC35 (red) were detected with a fluorescence microscope. (C) Colocalization of FAD24 with the nucleolus. HeLa cells transiently transfected with EGFP-FAD24 or EGFP-truncated FAD24 (FAD24 bZIP were fixed and green EGFP signal was detected with a fluorescence microscope. Bar in B, 10 µm for B and C.