Fig. 8. Localization of FAD24 in the presence of RNA polymerase II inhibitor. HeLa cells transiently transfected with EGFP-FAD24 were treated with -amanitin (50 µg/ml, 5 hours) and then fixed and stained with the nuclear speckle marker SC35. Fluorescence of FAD24 (green) and SC35 (red) were detected with a fluorescence microscope. Bar, 10 µm.