Fig. 3. Developmental expression of A. miniata SFK transcripts. A blot of 1 µg of polyA⁺ mRNA from A. miniata eggs (E) and embryos at different stages (B, blastula; G, gastrula) and bipinnaria larvae (Bi) was probed with the radiolabeled 5' UTR of AmSFK1 cDNA, the 3' UTR of the AmSFK2 cDNA or the 3' UTR of the AmSFK3 cDNA (top panels). The bottom panels are photographs of the stained RNA gel prior to transfer, demonstrating that each lane contained the same amount of polyA⁺ RNA.