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Fig. 5. Characterization of AmSFK proteins in eggs. Cytosolic (C) and membrane (M) fractions of A. miniata eggs (20 µg per lane) were separated on a 12% polyacrylamide SDS gel, transferred to nitrocellulose and probed with AmSFK1 IgY, AmSFK2 IgY or AmSFK3 IgY. Antibodies were detected with HRP-conjugated anti-chicken and ECL for a 30 second exposure. Arrows indicate the AmSFK proteins. Positions of molecular weight markers are indicated in kDa. The results are representative of nine experiments.