Fig. 7. Effects of injection of AmSFK SH2 domain proteins on Ca²⁺ release at fertilization. A. miniata oocytes were injected with the indicated protein mixed with Calcium Green dextran (CaG), matured and then fertilized. For each SH2 domain, 25 µM is equivalent to 1 mg/ml. Traces show Calcium Green fluorescence (y-axis) as a function of time (x-axis). Asterisks indicate the time of the fertilization potential, which marks sperm-egg fusion (McCulloh and Chambers, 1992; Swann et al., 1992).