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Fig. 3. Immunodetection of H3-diMeK9 on polytene chromosomes from larvae containing a heat-inducible Su(var)3-7 transgene. Green, anti-dimethyl-H3K9; white, DAPI staining. Homozygous HA:FL4D or HA:FL1A male or female larvae raised at 18°C (no hs) or submitted to daily heat-shocks at 35°C (hs). Arrows indicate the X chromosome.