Fig. 3. HeLa cells transfected with pEGFP-TPPP/p25 and their filamentous networks. (A-C) Cells were transfected and incubated for 20 hours, fixed with cold ethanol for immunocytochemistry to detect the alignment of TPPP/p25 (green) with tubulin (A), actin (B) and vimentin (C) (all red). (A) The microtubular network is not affected in the transfected cell, however, accumulation of fluorescent material started at the perinuclear position. Virtually complete colocalization with microtubules is observed. (B) Actin filaments show complete dislocation with the green EGFP-TPPP/p25 signal. Red and green images were taken at different positions on the z-axis to get focused signals. (C) Intermediate filaments (vimentin) do not show colocalization with EGFP-TPPP/p25. Bars, 10 µm.