Fig. 6. Transfected HeLa cells in the high expression state. HeLa cells were transfected with pEGFP-TPPP/p25 (A and B) or pcDNA3-TPPP/p25 (C). After 20 hours, cells were fixed with ice-cold buffered ethanol. Tubulin was stained with anti--tubulin antibody (red) (A and C). (B) Nuclei were stained with ethidium bromide (red). Protein construct was detected as green EGFP signal in A and B. Expressed TPPP/p25 was detected by FITC-conjugated secondary antibody in C. Bars, 10 µm.