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Fig. 2. p38 kinase is required for the inhibition of TNF{alpha}-induced filopodia formation. (A) p38 kinase assay in TNF{alpha}-treated MEFs. Serum-arrested cells were transfected with the HA-tagged p38 kinase, then treated with 100 ng ml–1 TNF{alpha} for the indicated times. Immunoprecipitated p38 kinase activity was determined as described in Materials and Methods. Values are the means±s.d. of three independent experiments. Statistical significance of p38 kinase activation caused by TNF{alpha} compared with untreated: P=0.061 (Student's t-test). (B) p38 kinase assay in transfected MEFs treated with TNF{alpha}. MEFs were transfected with the HA-tagged p38 kinase in the presence or not of vectors expressing either Cdc42-N17, N-Wasp, Cdc42-V12, MKK3, KD-MKK3 or pCDNA3. Serum-arrested cells were treated or not with 100 ng ml–1 TNF{alpha} for the indicated times. Immunoprecipitated p38 kinase activity was determined as described in Materials and Methods. The values are the means±s.d. of three independent experiments. Statistical significance of p38 kinase activation caused by TNF{alpha} compared with untreated: P=0.061 (Student's t-test).