JCS -- Kotzer et al. 117 (26): 6377 Figure IG7

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Fig. 7. Effect of fluorescent-tagged AtRabF2b on trafficking of aleu-GFP. Confocal images of tobacco leaf epidermal cells, 48-52 hours after Agrobacterium inoculation with aleu-GFP (OD₆₀₀ 0.1) and tagged-AtRabF2b (OD₆₀₀ 0.05). (A) With aleu-GFP alone, faint fluorescence is visible in some vacuoles. (B) Aleu-GFP and untagged AtRabF2b[S24N]. (C) Untransformed leaf. (D) Aleu-GFP (green) and YFP-AtRabF2b[wt] (red) expression, showing aleu-GFP is secreted to the apoplast. (E) Aleu-GFP (green) and YFP-AtRabF2b[S24N] (red) expression; aleu-GFP is secreted at similar levels to D. (F) aleu-GFP(green) and YFP-AtRabF2b[C198, 199S] (red) expression; aleu-GFP is not secreted to the apoplast. Bar, 50 µm.