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Fig. 1. Localization of endogenous NuMA in NRK 52E cells. (A) Early prophase. (B) Mid-prophase. (C) Late prophase. (D) Prometaphase. (E) Metaphase. (F) Anaphase. (G) Telophase. (H) Early G1. Immunostaining with anti-NuMA antibody (green), DNA is counterstained with Hoechst 33342. Maximal-intensity projections through the entire cell volume.