Fig. 2. Expression of EGFP-Rab27/Rab5 fusion proteins in AtT20 cells. Cells were transiently transfected with EGFP-Rab27 (A-C), EGFP-Rab5Q79L (D-F), EGFP-Rab5Q79L182/Rab2739 (G-L) or EGFP-Rab27187/Rab535 (M-R). After fixation, cells were permeabilised, and immunolabelled with antibodies to ACTH (B,H,N) or Tfn-R (E,K,Q). Panels on the left (A,D,G,J,M,P) show EGFP fluorescence and on the right (C,F,I,L,O,R) are the merged images.