Fig. 7. Transient over-expression in HeLa cells of several EGFP-Rab fusion proteins containing the hypervariable domain of Rab5a. HeLa cells were transfected with EGFP-Rab1a (A-C), EGFP-Rab1¹⁵⁶/Rab5⁵⁰ (D-F), EGFP-Rab2 (G-I) or EGFP-Rab2¹⁶⁰/Rab5⁴¹ (J-O). After 24 hours, cells were fixed, permeabilised and immunostained with an anti-Golgin97 antibodies (B,E,H,K), or Tfn-R antibody (N) as described in Materials and Methods. The panels on the left (A,D,G,J,M) show the EGFP signal and on the right (C,F,I,L,O) are the merged fluorescent signals.