Fig. 9. Expression of chimeric EGFP-Rab27 containing RabSF or RabF domains of Rab5 in AtT20 cells. Cells were transiently transfected with EGFP-Rab27/Rab5^SF2 (A-F), EGFP-Rab27/Rab5^SF3 (G-L) or EGFP-Rab27/Rab5^F4 (M-O). After fixation, cells were permeabilised and immunolabelled with antibodies to ACTH (B,H,N) or the Golgi 58K protein (E,K). Panels on the left (A,D,G,J,M) show EGFP fluorescence and panels on the right (C,F,I,L,O) are the merged images.