Fig. 3. Localization of GFP-SNX5_FL fusion protein in stably transfected HEK293 cells. (A) HEK293 cells stably expressing a GFP-SNX5_FL fusion protein were fixed in 4% paraformaldehyde, permeabilized with 0.1% Triton-X-100, and co-stained for EEA1 with human anti-EEA1 antibodies followed by human anti-Ig Alexa568. (B) High power image showing enlarged GFP-SNX5_FL-positive structures that also stain for EEA1. Note the lack of colocalization of the two molecules within these structures. Confocal images were collected with identical iris settings. Superimposed images (Merge) reveal regions of colocalization. Bar, 10 µm (A) and 5 µm (B).