Fig. 5. Endosomal localization of PX domain of SNX5 requires the activity of a wortmannin sensitive PI 3-kinase. (A) HeLa cells were transfected with GFP-SNX5_PX, and after 12 hours cell monolayers were serum-starved for 3 hours. Cells were either untreated or treated with 100 nM wortmannin for 2 hours at 37°C and fixed in 4% paraformaldehyde. (B) HEK293 cells expressing GFP-SNX5_FL were either untreated or treated with 100 nM wortmannin for 20 minutes at 37°C and fixed in 4% paraformaldehyde. Bar, 10 µm.