Fig. 8. SNX5 associates with the plasma membrane following EGF stimulation of transfected HeLa cells. HeLa cells were transfected with constructs encoding GFP-SNX5_PX or GFP-SNX5_FL. Untransfected HeLa cells were also grown under the same conditions. After 12 hours, cell monolayers were serum-starved for 3 h and either left unstimulated or stimulated with human EGF (100 ng/ml) for the indicated times, fixed and processed for fluorescence. The distribution of GFP-SNX5_PX and GFP-SNX5_FL was directly visualized by confocal microscopy. Untransfected cells were stained with anti-SNX1 monoclonal antibody followed by goat-anti-mouse IgG conjugated to Alexa568. Bar, 10 µm.