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Fig. 1. Identification of sem1 mutants in Saccharomyces cerevisiae. (A) Isolation of sem1 mutants. Two sem1 mutants were identified as suppressors of Dsk2-mediated lethality. Both the sem1 point mutants and sem1{Delta} were temperature-sensitive for growth. (B) Mutation sites of sem1 mutant alleles. Mutated sites (sem1-60 and sem1-64) are indicated in italics (60W and 64W). (C) Loss of Sem1 protein in sem1 mutants. Sem1 protein was detected in cell extracts by immunoblotting with anti-Sem1 antibody (top); Cdc28 was detected (bottom) as a loading control.