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Fig. 2. RT-PCR of purified mouse RPE cells. (A) Representative RT-PCR of mRNA expression of Rab27a, Rab27b, Melanophilin (Mlph), Myrip, Myo7a and Myo5a in purified mouse RPE cells, retina and cerebellum. Control, PCR reaction without added DNA. The position of DNA size-standards in bp is given at the left. (B) Representative RT-PCR of mRNA expression of different exophilins in purified mouse RPE cells, retina and cerebellum. 1, Exophilin 1-Rabphilin 3a; 2, Exophilin2-Granuphilin-Sytl4; 3, Exophilin3-Melanophilin-Slac2-a; 4, Exophilin4-Sytl2; 5, Exophilin5-Slac2-b, 6, Exophilin6-Sytl3; 7, Exophilin7-Sytl1; 8, Exophilin8-Myrip-Slac2-c; 9, Exophilin9-Sylt5. Control, PCR reaction without added DNA. The position of DNA size-standards in bp is given at the left.