Fig. 1. The PI3-kinase inhibitor LY294002 affects phagocytosis. (A) Wild-type AX3 cells were incubated with TRITC-labelled yeast and at the indicated time points the fluorescence of internalised particles was measured. Average and standard deviation of three independent experiments are shown. (B) Uptake of fluorescent 1 µm beads by AX3 cells. Average and standard deviation of three independent experiments are shown. (C) Effect of LY294002 treatment on phagocytosis of bacteria by AX3 cells. The internalisation of bacteria was measured as a reduction of the optical density at 600 nm, here plotted as a percentage of the OD₆₀₀ at t=0 hours. The curve `bacteria' refers to a control with bacteria but without Dictyostelium cells. The average and standard deviation of three independent experiments are shown.