Fig. 10. 3-Phosphoinositide distribution during aggregation of Dictyostelium cells. (A) GRP1-PH-GFP localisation in aggregation stream cells moving towards a cAMP signalling centre (arrow indicates direction of movement). See also supplementary material Fig. S7a. The white square marks the position of the cells shown in (B). (B) Pseudocolour representation of the cell from (A) showing small transient changes in membrane binding (red: high fluorescence intensity; blue: low intensity). (C) Periodic changes in fluorescence intensity as measured in the centre of the aggregation stream. (D) CRAC-PH membrane localisation is restricted to the front of the cells in aggregation streams. See also supplementary material Fig. S7b. (E) Close-up of the cells marked in (D) showing the changes in CRAC-PH localisation. (F) Measurement of the periodic changes in CRAC translocation in the aggregation stream. (G) Cells expressing DAPP1^G176A-PH-GFP moving towards an aggregation centre on the right-hand side (indicated by large arrow). The small arrows mark the membrane localisation of the probe in cells that seem to engulf the cells in front. (H) Close-up of the cell marked in (G) showing the engulfment of another Dictyostelium cell (asterisk) accompanied by a transient rise of PtdIns(3,4)P₂ levels at the phagosome. See also supplementary material Fig. S7c. Bars, 50 µm.