Fig. 4. Quantitative analysis of membrane localisation during phagocytosis. Fluorescence was measured at the plasma membrane (p) at the phagocytic cup/phagosome as well as in the adjacent cytoplasm (c), as indicated in inset in (A), and the ratio calculated as described in experimental procedures. Curves were aligned using `phagosome closure', the formation of a membrane enclosed phagosome as t=0 seconds. Data were averaged from 10 (GRP1), 13 (TAPP1), 7 (CRAC-PH), 9 (DAPP1-PH) and 12 (DAPP1^G176A) cells.