Fig. 5. Localisation of f-actin specific probes during phagocytosis. (A) Coronin-GFP localises to the site of particle engulfment, followed by a complex redistribution process (25-45 seconds) until the phagosome is propelled into the cytosol. Yeast marked by asterisks. Bar, 5 µm. See also supplementary material Fig. S2. (B) The f-actin specific probe ABD-GFP shows a similar localisation pattern during the uptake of a yeast cell (marked by asterisks). Bar, 5 µm. (C) Correlation of phagosome movement with changes in GRP1/TAPP1 binding to phagosomal membranes. The averaged fluorescence intensity data for GRP1 and TAPP1 are taken from Fig. 4. The movement of yeast particles during engulfment was tracked and the average velocity from five phagocytosis events from different cells plotted. Phagosome closure occurs at t=0 seconds.