Fig. 2. VEGF increased adhesion of HUVEC to collagen type I. (A) HUVEC were starved for 18 hours and then plated on 96-well plates (5.0x10⁴ cells/well). VEGF was added to the medium 10 minutes before plating and the adherent cells were measured by WST-1 assay, as described in Materials and Methods. (B) HUVEC were transiently transfected with the indicated plasmids 18 hours before VEGF stimulation. LY294002 was added 30 minutes before the stimulation. Data are expressed as the mean ± s.d.