Fig. 8. Effects of ILK-KD and LY294002 on chemotactic migration of HUVEC stimulated by VEGF. HUVEC were transiently transfected with the indicated plasmids 18 hours before VEGF stimulation. LY294002 was added to the upper chamber containing HUVEC 30 minutes before the stimulation. rhVEGF was added to the lower chamber at a concentration of 30 ng/ml and migratory activity of the cells was estimated, based on the number of cells migrating to the lower chamber. Data are expressed as the mean ± s.d.