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Fig. 3. Anx2 overexpression results in an increase in ALP activity. (A) ALP protein was detected by immunofluorescence in SaOSLM2 cells overexpressing anx2. (B) Cell-associated ALP activity was measured in osteoblast-like cells cultured in both non-differentiation-(white bars) and differentiation-inducing (black bars) media. * Statistically significant increases in ALP activity by culturing the cells under differentiation conditions (P<0.05). #Statistically significant increases in ALP activity induced by anx2 overexpression (P<0.05).