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Fig. 6. Anx2 and ALP are associated with lipid rafts. (A) Lipid raft microdomains were isolated from SaOSLM2 and SaOSLM2-anx2GFP cells by centrifugation through a 5-40% sucrose gradient. Fractions were collected and insoluble material was pelleted from each fraction in which the sucrose had been reduced. Anx2 was detected by immunoblot analysis. (B) ALP activity was evaluated in the lipid raft fraction pellets isolated from SaOSLM2 (solid line) and SaOSLM2-anx2GFP (dashed line) cells. The * in fraction 6 represents statistical significance between anx2-overexpressing ALP activities and parental ALP activities (P<0.05).