Fig. 1. Comparison of a matrix protein and a non-matrix protein in interphase and metaphase cells. Interphase (A-C) and metaphase (D-F) NRK cells were costained for mannosidase II (A,D) and GM130 (B,E). Single-channel images are also overlayed (C,F) with mannosidase II in green and GM130 in red. The images shown are 2D projections from the deconvolved 3D data set using summation of pixel values in each plane. Bar: 10 µm.