Fig. 4. Coincidence of Golgi proteins in mitotic Golgi clusters. Metaphase NRK cells were costained with antibodies against mannosidase II and GRASP65 and a merged image is shown with mannosidase II in green and GRASP65 in red. Metaphase cells were also costained with antibodies against TGN38 and giantin, and the merge shows TGN38 in green and giantin in red. All images shown are summed projections that were thresholded to isolate mitotic Golgi cluster staining from vesicular haze. Note that markers were frequently adjacent in clusters rather than truly coincident. Bar: 10 µm.