Fig. 5. Matrix proteins are selectively, but incompletely, retained in BFA remnants. NRK cells, either untreated (A,B) or treated with BFA for 30 minutes (C,D), were costained for mannosidase II (A,C) and GM130 (B,D). Summed projections are shown. Note that for an unexplained reason, nucleolar-like staining was observed when the anti-mannosidase II antibody was used to stain interphase cells, particularly at the exposure setting necessary to visualize BFA-treated cells. This non-specific staining was excluded in quantitative analyses. Bar: 10 µm. (E) Untreated interphase (open bars) and BFA-treated NRK cells were stained for different proteins as indicated, and the percentage fluorescence in objects of each was calculated as in Materials and Methods (±s.e.m.). The results are averages from 10 cells per condition.