Fig. 3. YY1 mRNA and protein levels throughout the cell cycle. Numbers above the lanes indicate cell-harvest time points (hours after shake-off). (A) RNA gel blots of equal amounts of total RNA from synchronized CHO cells were incubated with radiolabeled cDNA clones for YY1, then reprobed with EF1- as a loading control. (B) Protein gel blots were incubated with anti-YY1 antibodies (YY1) or anti--tubulin (tubulin) and detected by ECL.