Fig. 7. Change in YY1 subcellular localization in HeLa cells. Synchronized HeLa S-3 cells were collected by mitotic shake-off at the time points indicated. Fixed cells were stained for YY1, imaged and presented as described in Fig. 4. Color-merge images show the nucleus (red, DAPI) and YY1 (green); dashed lines indicate the cell periphery. At late mitosis (A) and 1 hour after shake-off (B), YY1 appears mainly cytoplasmic. After 3 (C) and 5 (D) hours, most of the YY1 signals are located in the nucleus, where they appear as yellow-green in the merged images because of colocalization with red DAPI signal. Scale bars: 2 µm.