Fig. 11. Influence of high levels of Clb2 in mating and pathogenicity. (A) Disease symptoms on the leaf blades of young maize plants 14 days post inoculation with the strains indicated. Observe the anthocyanin production (arrow a) and small tumors (arrow t) in the wild-type infection and the chlorosis (yellowing of the green tissue of the leaf, arrow c) in the mutant infection. (B) Mating assay of Clb2-overexpressing cells and wild-type strains. The appearance of white filaments indicates formation of dikaryotic hyphae. Note that the mutant cross produced fewer hyphae. (C) Dikaryotic hypha resulting from a wild-type FB1 x FB2 cross. Note the empty compartments to the right of the elongated tip cell. (D) Dikaryotic hypha resulting from a mutant TAU26 x FB2 cross. Note the `spiky' appearance of the elongated tip cell. (E) Hyphal development of wild-type cells inside the plant tissue. Cross walls separate the long cylindrical cells of hyphe. The inset shows a lower magnification of the filamentous network. (F) Hyphal development of mutant cells inside the plant tissue. These cells appeared to generate incipient branches at a higher frequency than wild-type cells. Scale bars: 50 µm (B,C); 20 µm (E-F).