Fig. 7. Consequences of Clb1 overexpression. (A) Cell morphology of TAU36-1 cells growing in YPA (inducing conditions). Observe in the cells stained with DAPI the diverse intensity of fluorescence from various nuclei. Scale bar: 10 µm. (B) Flow cytometry analysis of wild-type (FB1) and TAU36-1 cells grown in no-induction (YPD) and induction (YPA) conditions. Cells grown in YPD were centrifuged, washed twice in water and resuspended in the appropriated medium. Samples were taken for FACS analysis at the indicated times. Note the peaks with DNA content below 1C and above 2C. (C) Serial tenfold dilutions of exponential cultures in YPD of wild-type FB1 and TAU36-1 (FB1 Pcrg-clb1) cells were spotted on induction plates (YPA, YP medium with 2% arabinose) with 5 µg/ml phloxin B and incubated at 28°C for 4 days. Phloxin B accumulates in dead cells and subsequently red colonies are indicative of a loss of viability. (D) Benomyl sensitivity of strain overexpressing Clb1. Serial tenfold dilutions of exponential cultures of FB1 and TAU36-1 cells were spotted on induction plates containing 0.6 µg/ml of benomyl. Plates were incubated at 28°C for 4 days.