Fig. 1. Effect of FGF2 and PMA on Cx43 phosphorylation on S262. Western blots of total lysates from cardiomyocyte cultures (20 µg/lane) probed with antibodies recognizing (A) total, or (B) P-262-Cx43, as indicated. Cultures were stimulated with FGF2 (10 ng/ml) or PMA (100 nM) for 15-60 minutes, as indicated.