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Fig. 4. Effect of 262A- and 262D-Cx43 overexpression on cardiomyocyte anti-Ki-67 labelling. Myocyte cultures infected with adenoviral vectors expressing (A) 262A-Cx43 or (B) 262D-Cx43 were labelled for N-cadherin (green; rabbit polyclonal antibodies to delineate cell-cell contact cardiomyocyte areas), Ki-67 (green; rabbit polyclonal to detect nuclei of proliferating cells; elicits speckled nuclear staining) and Cx43 (red; monoclonal antibodies). Arrowheads, non-proliferating myocytes; arrows, proliferating myocytes. Scale bar: 50 µm.