Fig. 6. Effect of Cx43 and S262 mutations on dye coupling. (A,B) Representative micrographs of dye migration (green), assessed by scrape-loading, in confluent cultures (seen by simultaneous phase contrast photography) expressing (A) 262A- or (B) 262D-Cx43. Arrowheads indicate length of dye spreading. In C dye spreading is plotted as a function of overexpressing wt-, 262A- (+/–PMA) and 262D-Cx43 (+/–PMA), compared to vector-infected cultures, as indicated (n=4). Bars indicate s.e.m.