Fig. 8. Northern blot analyses of sams1 and sams2 genes following infection of D amoebae with X-bacteria. Total RNAs (20 µg) isolated from amoebae collected every 4 days after infection were separated in formamide gels and then transferred to nylon membranes. The signal was detected subsequently on the same blot with ³²P-labeled sams1, sams2 and myosin probes. The myosin probe was used as a loading control.