Fig. 6. Localization of cytoskeletal proteins in DEP-1-expressing cells. Control 1A2, or DEP-1-expressing 2C2 or 2D3 cells were induced with mifepristone for 24 hours, plated on to coverslips and stained for F-actin, tubulin, vinculin or paxillin as described in Materials and Methods. All of the DEP-1-expressing cells showed disruption of microfilament bundles, microtubules and adhesion plaques. Scale bar: 10 µm