Fig. 8. Association of Hsp90 and Hsc70 with denatured myosin S1. The myosin S1 proteolytic fragment of pectoralis muscle myosin was denatured with 6.5 M guanidine HCl and rapidly diluted into C2C12 S30 fraction. After a short incubation at room temperature, the S1 and associated S30 proteins were immunoadsorbed to beads coated with an anti-S1 mAb. The immunopellets were resolved on 10% SDS-PAGE and stained or immunoblotted with Hsp90 and Hsc70 specific antibodies. The Hsp90 antibody detects an 86 kDa band in the complete reaction containing denatured myosin S1. Similarly, the Hsc70 antibody detects a 70 kDa band in this reaction. The Hsp90, and Hsc70 antibodies identify single bands of sizes 86 kDa, and 70 kDa respectively in the S30 used here as positive control.