Fig. 3. Flow cytometry analysis of Q267E NIS. Non-permeabilized (A,C) or permeabilized (B,D) COS-7 cells not-transfected (black dotted line) transfected with WT hNIS (red line) or hQ267E NIS (green line) were incubated with 4 nM anti-Ct-hNIS Ab (A,B) or with a 1:50 dilution of anti-hNIS VJ1 Ab (C,D), followed by incubation with fluorescein-conjugated goat anti-rabbit Ab (A,B) or fluorescein-conjugated anti-mouse Ab (C,D). Aliquots containing 2x10⁶ cells were subjected to flow cytometry.