Fig. 6. Immunofluorescence analysis of COS-7 cells transfected with WT hNIS or hQ267E NIS. Non-permeabilized (top panels) or permeabilized (bottom panels) transfected COS-7 cells were incubated with 4 nM anti-Ct-hNIS Ab (A-D) or with anti-hNIS VJ1 Ab (1:50) (EH). A second incubation was performed with fluorescein-conjugated goat anti-rabbit Ab (A-D) or fluorescein-conjugated anti-mouse Ab (E-H). The upper rectangular panels show xz cross-sectional images obtained using a confocal microscope.