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Fig. 1. Assay for cell branching in fission yeast. cdc10-129 mutant cells are arrested in G1 by a temperature shift, drugs are added, and the temperature is subsequently shifted down to allow cells to reenter the cell cycle. Cells are shown 2 hours after release from cell-cycle arrest, in the presence of (A) control DMSO or (B) 100 µg/ml TBZ.