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Fig. 4. TBZ but not MBC causes transient depolarization of the cortical actin cytoskeleton and arrest of cell elongation. (A) Percentage of cdc10-129 arrested cells with a polarized actin cytoskeleton 45 minutes after addition of DMSO, TBZ, MBC or TBZ plus MBC. (B) Time-course showing the percentage of cdc10-129 arrested cells with polarized ral3-GFP, after addition of TBZ, MBC or TBZ plus MBC. (C) Mean cell length versus time in a cdc10-129 block-and-release experiment, using cells treated either with DMSO, TBZ or MBC. (D) Mean cell length versus time in wild-type elutriated cells treated either with DMSO, TBZ or MBC. (E-H) Images of cdc10-129 cells showing Rhodamine-Phalloidin staining of (E) polarized actin and (F) depolarized actin, and live-cell fluorescence of (G) polarized ral3-GFP and (H) depolarized ral3-GFP.