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Fig. 7. Transient depolymerization of the actin cytoskeleton by latrunculin B (LatB) triggers microtubule-dependent branching in cdc10-129 tea2-1 mutants in a tea1+ background but microtubule-independent branching in a tea1{Delta} background. Percent branched cells in cdc10-129 block-and-release experiments after either a LatB pulse followed by wash-out or a LatB plus MBC pulse followed by wash-out into MBC. (A) cdc10-129 tea2-1, (B) cdc10-129 nda3-TB101 tea2-1, (C) cdc10-129 tea1{Delta} tea2-1, (D) cdc10-129 tea1{Delta}.