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Fig. 8. Tea1p is required for a high frequency of branching after a mild depolarization of the cortical actin cytosksleton. (A) Temperature shift in mod5{Delta} tea2-1 double mutants produces a synergy in cell branching that depends on tea1p. The percentage of branched cells in the indicated strains is shown 2 hours after shift-up from 32°C to 36°C. (B) Percentage of branched cells in a cdc10-129 block-and-release experiment, using a TBZ wash-out (Fig. 5) and either cdc10-129 nda3-TB101 tea2-1 triple mutants or cdc10-129 nda3-TB101 tea1{Delta} tea2-1 quadruple mutants.