Fig. 1. The subcellular localization of mSTI1-EGFP and NLS^mSTI1-EGFP. (A) NIH3T3 fibroblasts were transiently transfected with pB-mSTI1-EGFP, fixed in 3.7% paraformaldehyde, permeabilized in 0.1% saponin (all panels) and immunostained with SF1 polyclonal rabbit anti-mSTI1 and Cy3-conjugated donkey anti-rabbit antibodies (middle and right panel). Cells were mounted and visualized by confocal laser fluorescence microscopy. Scale bars, 10 µm. (B) NIH3T3 fibroblasts were stably transfected with pB-EGFP (top), pB-mSTI1-EGFP (middle) and pB-NLS^mSTI1-EGFP (bottom). Cells were fixed in 3.7% paraformaldehyde and stained with DAPI before visualization by confocal laser fluorescence microscopy. Scale bars, 10 µm.