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Fig. 2. FACS analysis before and after PI-PLC treatment. FACS profiles giving CEA and CC1-mutant cell surface expression on LR-73 transfectant cells with and without treatment with PI-PLC to remove GPI-anchored molecules. Profiles for CEA (positive GPI-linked control), CC1-4L (negative TM-linked control) and Neo (background control) transfectants are also shown.