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Fig. 3. GPI-processing evaluation of various CC1-4L mutants by cold nonionic detergent solubility assay. Immunoblot analysis of cold Triton X-100 extracts of LR-73 transfectant cells. Neo transfectants and naturally occurring CEA family members, CEA, CEACAM6 (GPI-linked), and CC1-4L (TM-linked) were used as controls. a-d represent results for four separate immunoblots.